Tubes

Invert 3 to 4 times tube must be filled completely

2.7mL sodium citrate tube with light blue top
Panel Name
Lupus Anticoag Rflx Confirm (6B)
Miscarriage Profile (4B, 2ST & 1L)
 
2.7mL sodium citrate tube with light blue top
Test Name
Fibrinogen
Prothrombin Time (PT)
PTT

Invert 5 times

blood should clot upright for 30 minutes but no longer than 2 hours prior to separation

Sterile collection tube for serum separation
Panel Name
Acute HepatitisPanel

Basic Metabolic Panel

Comprehensive Metabolic Panel

Electrolyte Panel

Iron and Iron Binding Capacity

Lipid Panel

Miscarriage Profile (4B, 2ST & 1L)

Obstetric Panel (1L & 2ST)

Sterile collection tube for serum separation
Test Name

17- OH Progesterone 

ANA (Anti-Nuclear Ab w/ Reflex Titer)

ANA Reflex Autoimmune Ab Profile

ANA Reflex Nuclear Ab Profile

Cancer Antigen 19-9

Cancer Antigen 15-3

Cancer Antigen 125

Cardiolipin IgG/IgA/IgM

C-Reactive Protein

Creatinine Clearance, 24 HR (Submit 24 Hr Urine Jug in conjunction with Serum Separator Tube)

CRP, High Sensitivity

Cytomegalovirus IgG/IgM Panel

DHEA Sulfate
Estradiol
Estrogen Fractionated
Ferritin
Follicle Stim Hormone

Folate

Fructosamine
HCG, Qualitative

HCG, Quantitative

Hepatitis B Surface Ab
Hepatitis B Surface Ag
Hepatitis C Anitbody
Hepatitis C Refliex Quantitative

HIV 1/2 Ab/Ag Combo

HSV 1&2 IgG

Insulin
Luteinizing Hormone (LH)
Parvovirus B19 IgM
Progesterone
Prolactin
Quadruple Screening Test w/ Interpretation

Rheumatoid Factor

RPR Reflex Titer
Rubella IgG
Screen, First Trimester
Sequential ScreenSM , First Trimester
Sequential ScreenSM , Second Trimester

Treponema Ab Rflx RPR

T3, Free
T4, Free

Testosterone, F/T/SHBG

TSH
TSH w/ Reflex to Free T4
Uric Acid
Varicella Zoster IgG
Vitamin B-12
Vitamin D, 25, OH
2nd AFP-NTD Screen

Invert 8 to 10 times

 

4.0mL Potassium EDTA tube with lavendar top

 

Profiles & AMA Panel

Obstetric Panel (1L & 2ST)

Test Name
BRCA 1 and BRCA2 Comprehensive
Fragile X with Reflex Methylation
Spinal Muscular Atrophy (SMA)
Antibody Screen
Blood Group (ABO) and Rh Type
CBC w/ Auto Diff w/ Platelets
CBC w/o Diff w/ Platelets
Cystic Fibrosis Genotyping, 139 Mutations
Cystic Fibrosis Genotyping, 39 Mutations
Factor V Leiden
Glucose-6-PD Quant
Hemoglobin A1c
Hemoglobin Electrophoresis
Sedimentation Rate (ESR)
Sickle Scrn Rflx Elect

Invert 8 to 10 times

do not confuse with urine culture tube

Sodium Flouride collection medium with grey top tube
Panel Name
Glucose
Glucose, 1HR, Gestational Screen, 50gm Load
Glucose Tolerance, 2 HR Gestational, 75gm Diagnostic
Glucose Tolerance, 2 HR, ADA 75grm Load
Glucose Tolerance, 3 HR Gestational, Diagnostic 100gm Load

Swabs

Aptima SimpleSwab transport medium with swab included
Test NameSpecimen Requirements

Bacterial Vaginosis
Candida
HSV 1/2
Trichomonas

Vaginal swab collected in Aptima vaginal swab transport tube (orange Aptima tube).

  1. Vaginal swab collected in Aptima vaginal swab transport tube (orange Aptima tube). Partially peel open the swab package. Remove the swab. Do not touch the soft tip or lay the swab down. If the soft tip is touched, the swab is laid down, or the swab is dropped, use a new Aptima vaginal swab specimen collection kit.

  2. Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line. Do not hold the swab shaft below the score line.

  3. Carefully insert the swab into the vagina about 2 inches (5 cm) past the introitus and gently rotate the swab for 10-30 seconds.

  4. Make sure the swab touches the walls of the vagina so that moisture is absorbed by the swab and then withdraw the swab without touching the skin.

  5. While holding the swab in the same hand, unscrew the cap from the tube. Do not spill the contents of the tube. If the contents of the tube are spilled, use a new Aptima vaginal swab or specimen collection kit.

  6. Immediately place the swab into the transport tube so that the score line is at the top of the tube. 
  7. Carefully break the swab shaft at the score line against the side of the tube. Immediately discard the top portion of the swab shaft. Tightly screw the cap onto the tube.
  8. Submit the sample to the laboratory at Room Temperature or Refrigerated.

CT/NG

Female endocervical or vaginal swab or male urethral swab in Aptima transport tube. Room Temperature.

Aptima collection tube for urine testing, includes 2.0mL tune and pipette
Test NameSpecimen Requirements
CT/NG

First 20-30 mL voided urine. The patient must not have urinated for the last 1 hour. Transfer 2 mL urine to Hologic Aptima urine collection tube within 24 hours of collection. Room Temperature. Male or female samples accepted.

Trichomonas

  1. Patient must not have urinated for at least 1 hour prior to specimen collection. Direct patient to provide a first-catch urine (approximately 20 to 30 mL of the initial urine stream) into a urine collection cup free of preservatives.

  2. Collection of larger volumes of urine may result in rRNA target dilution that may reduce test sensitivity.

  3. Female patients should not cleanse the labial area prior to providing the specimen. Remove the cap and transfer 2 mL of urine into the urine specimen transport tube (yellow Aptima tube) using the disposable pipette provided within 24 hours of collection.

  4. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine specimen transport tube label.

  5. Re-cap the urine specimen transport tube tightly. Submit specimen to the laboratory at Room Temperature or Refrigerated. 

Note: male specimens will not be accepted.

BD Affirm-VP ambient temperature transport medium with swab
Test NameSpecimen Requirements

Vaginal Pathogens DNA Panel

Vaginal fluid collected in BD Affirm VPII ambient temperature transport system. Room Temperature. Note: separate swabs should be submitted if other tests are also ordered (e.g., Culture).

Trio of eSwab collection devices in white, green and blue
Test NameSpecimen Requirements
Routine, CultureBacterial culturette swab inoculated with infected material. Room Temperature.
Genital, CultureInoculated swab in transport media. Room Temperature.
Culture, Grp B Strep
Culture, Grp B, Pen. Aller
Inoculated swab from vaginal/rectal source in transport media. Room Temperature.

Vials

The ThinPrep PAP test 20mL Transport medium
Test NameSpecimen Requirements
Pap Test, Image Guided, Pap, Age-Based with DNA HPV, Pap, Age-Based with mRNA HPV

Obtain an adequate sampling from the cervix using spatula, brush, or broom. Insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix. For collection with broom: push gently and rotate in a clockwise direction five (5) times. For collection with brush: slowly rotate . 1/4 - 1/2 turn in one direction. Rinse the broom into the vial by pushing the broom into the bottom of the vial ten (10) times, forcing the bristles apart. As a final step, swirl the broom vigorously to further dislodge material. Discard the collection device. Do not leave collection device in the vial. Remove immediately. Tighten the cap so that the torque line on the cap passes the torque line on the vial. Record the patient's name and ID number on the vial. The sample will be returned if it is received without proper labeling. Record the patient name and medical history on the cytology requisition. Submit the sample to the laboratory at Room Temperature. Medicaid THSTEPS samples must sent to DSHS laboratory.

CT/NG
CT (Chlamydia)
NG (Gonorrhoeae)

 

1 mL fluid from Preservcyt vial (ThinPrep). Room Temperature.

HSV 1/2, Trichomonas

1 mL ThinPrep (Preservcyt) solution. Room Temperature or Refrigerated.

HPV, Non-reflexThinPrep Preservcyt solution. Room Temperature (15-25 °C).
HPV, Rflx, if ASC-USIndicates request for high risk HPV if ASCUS ThinPrep result. When reflex criteria are met, code 8038 will be added.
HPV, Rflx, if ASC-US/LSILIndicates request for high risk HPV if ASCUS or LGSIL ThinPrep result. When reflex criteria are met, code 8038 will be added.
HPV, Rflx, if Epith AbnIndicates request for high risk HPV if any abnormal ThinPrep result. When reflex criteria are met, code 8038 will be added.
Bacterial Vaginosis Candida6 mL ThinPrep solution. Room Temperature or Refrigerated.
SurePath liquid based pathology transport medium
Test NameSpecimen Requirements
Pap Test, Image Guided, Pap, Age-Based with DNA HPV

Obtain material with spatula, endocervical brush, or cervical broom. Insert the brush or broom into the endocervical canal. Apply gentle pressure on the cervix until the lateral bristles of the brush bend against the ectocervix. Maintaining gentle pressure, hold the stem between the thumb and forefinger and rotate the broom five (5) turns clockwise OR slowly rotate the brush 1/4 -1/2 turn in one direction.

The entire head of the brush/broom is removed from the handle and placed into a SurePath collection vial. The vial is capped, labeled and sent with appropriate paperwork to the laboratory for processing. The vial must be labeled with patient name or it will be returned. Room Temperature. Medicaid THSTEPS samples must sent to DSHS laboratory.

CT/NG

CT (Chlamydia)

NG (Gonorrhea)

1 mL fluid from SurePath vial. Room Temperature

HPV, Non-reflexSurePath Preservative fluid solution. Room Temperature (15-25 °C).
HPV, Rflx, if ASC-USIndicates request for high risk HPV if ASCUS SurePath result. When reflex criteria are met, code 8037 will be added.
HPV, Rflx, if ASC-US/LSILIndicates request for high risk HPV if ASCUS or LSIL SurePath result. When reflex criteria are met, code 8037 will be added.
HPV, Rflx, if Epith AbnIndicates request for high risk HPV if any abnormal SurePath result. When reflex criteria are met, code 8037 will be added.

HSV 1/2

Trichomonas

1 mL SurePath solution. Room Temperature or Refrigerated.

Other

Polymedco OC Auto
Test Name
Occult Bld, Fecal, Immunoassay Diagnostic
Occult Bld, Fecal, Immunoassay MC Screen
Sterile container for collecting stool and other materials
Test Name
Drug Abuse Panel 10 w/ Oxycodone
Fungus non-blood or Skin, Culture
Microalbumin/Creatinine, Random and Ratio

*Urine specimens 

Urine Culture tube with grey top 4 mL
Test Name
Urinalysis (submit Urine Tube in conjunction with Urine Culture)
Urine,  Culture
Urine, Pen Allergic, Culture
Jug for collection of urine for testing over a 24-hour period
Test Name
Protein, 24 HR Urine
Creatinine Clearance, 24 HR
Urine collection tube
Test Name
Urinalysis w/ Reflex Micro
Urinalysis (submit Urine Culture in conjunction with Urine Tube)
Rafid FFN tube for collection of specimen
Test Name
Fetal Fibronectin
Cellfree
Test Name
NIPT
NIPT with SexChromosome Aneuploidies
NIPT with Monosomy X
NIPT with 22q
NIPT with Sex Chromosome Aneuploidies & 22q
NIPT with Monosomy X & 22q