Collection Guides are organized by Collection Containers

Cary Blair with indicator add specimen to transport for in vitro diagnosis
Test Name Transport TempSpecimen RequirementsRejection Criteria

Stool Culture (includes Campylobacter)

Vibrio Culture

Yersinia Culture

E. Coli 0157:H7 Culture with Shigatoxin

Refrigerated (2-8 °C)Fresh stool in Cary-Blair transport media. No more than two bacteriology specimens are recommended. Two specimens should be submitted on separate days to increase the probability of recovering pathogens. Two separate specimens will pick up 99% of bacterial pathogens. If Vibrio or Yersinia is suspected, codes specific for those tests should be ordered.Reject due to contamination on outside of container, samples submitted in a diaper or tissue paper, dry swabs, frozen specimens, specimen submitted in fixative (formalin or pva), expired transport media, swab not submitted in transport media, viral transport media, and stool transport exhibiting yellow colored solution (beyond buffering capacity).
ShigatoxinRefrigerated (2-8 °C)Fresh stool in Cary-Blair transport media.Reject due to contamination on outside of container, samples submitted in a diaper or tissue paper, dry swabs, frozen specimens, specimen submitted in fixative (formalin or pva), expired transport media, swab not submitted in transport media, viral transport media, and stool transport exhibiting yellow colored solution (beyond buffering capacity).
Stool pathogen Panel by PCRRefrigerated (2-8 °C)2 mL liquid stool in Cary-Blair transport media.Testing will only be performed on liquid stools. Formed stools will be rejected.
Sterile container for collecting stool and other materials
Test NameTransport TempSpecimen RequirementsRejection Criteria
C.difficile GDH Reflex Toxin/PCRRefrigerated (2-8 °C)10 mL fresh stool. Optimal specimen less than 24 hours.  If specimen cannot be tested within 72 hours, freeze.Room temperature samples, swabs, sample submitted in preservative or additives, tissue paper or diapers, viral transport media, leaking containers or cary blair transport media.
C.difficile Toxin by PCRRefrigerated (2-8 °C)Collect fresh diarrheal (unformed) stool, unless ileus due to Clostridium difficile is suspected, in a clean, leakproof stool collection container without media or preservative. Testing will only be performed on liquid stools. Formed stools will be rejected.
H. pylori AntigenRefrigerated (2-8 °C)10 grams unpreserved stool. Performance characteristics have not been established for watery, diarrheal stools. To avoid false Negative results, no antibiotics, proton pump inhibitors, or bismuth preparation should be administered to patients for 14 days prior to sample collection. Current medical practice recommends that assessment of eradication by any diagnostic method should be done at least 4 weeks following completion of therapy. Performance characteristics have not been established in asymptomatic populations.Room temperature samples, swabs, sample submitted in preservative or additives, tissue paper or diapers, viral transport media, leaking containers or cary blair transport media.
OsmolalityCritical Frozen (CFZ)5 mL unpreserved liquid stool. Sample must be liquid. Do not add saline or water to liquefy sample. When multiple tests are ordered, submit separate sample for this test.Formed stool and samples submitted in media or preservative.
PorphyrinsCritical Frozen (CFZ)5 grams random stool sample. Must be formed sample. Protect from light. Freeze immediately. When multiple tests are ordered, submit separate sample for this test.24 hour collection, thawed samples or liquid samples.
Electrolytes (Sodium, Potassium and Chloride)Frozen (≤ -20 °C)5 grams 24 hour or random stool. Sample must be liquid. Do not add saline or water to sample to liquefy. When multiple tests are ordered, submit separate sample for this test.Non-liquid stool, room temperature samples or viscous samples.
MagnesiumFrozen (≤ -20 °C)5 gm aliquot from well mixed 24 hr or random stool collection. Sample must be liquid. Do not add saline or water to liquify sample. Record weight of sample and time of collection. When multiple tests are ordered, submit separate sample for this test.Formed stool or viscous samples.
Pancreatic ElastaseFrozen (≤ -20 °C)5 grams random, unpreserved stool. Interruption of enzyme substitution therapy is recommended in order to avoid the possibility of cross-reaction with porcine enzymes. When multiple tests are ordered, submit separate sample for this test.Stool submitted in transport media or preservative.
SodiumRefrigerated (2-8 °C)5 gram liquid stool. Do not add saline or water to liquify sample.Formed stool or viscous samples.
Parasite Antigen Profile
(E.histolytica AG, Cryptosporidium AG, Giardia Specific AG)
Refrigerated (2-8 °C)1 gram fresh stool.Room temperature samples, swabs, sample submitted in preservative or additives, tissue paper or diapers, viral transport media, leaking containers or cary blair transport media.
LeukocytesRefrigerated (2-8 °C)5 mL unpreserved stool or colon washings. WBC's can be read from O and P examination. Unpreserved samples greater than 24 hours, specimen submitted in stool transport media, tissue paper or diapers, leaking containers or frozen samples.
pHRefrigerated (2-8 °C)1 mL fresh unpreserved stool.Sample submitted in preservative or sample containing residual detergent.
Fecal Fat, QualitativeFrozen (≤ -20 °C)5 grams random stool in a stool collection container with no preservative. Freeze within 1 hour of collection. When multiple tests are ordered, submit separate sample for this test.Sample submitted in preservative or diaper.
Plastic pre-weighted collection container for collecting stool
Test NameTransport TempSpecimen RequirementsRejection Criteria
Fecal Fat, QuantitativeFrozen (≤ -20 °C)Collect 24, 48, or 72-hour stool collection. Refrigerate sample during collection. Record weight of final collection. Mix well and remove aliquot. If weight and time are not provided, it is assumed that the collection is random and alternative testing will be performed. Freeze within 1 hour of completion of sample collection. The patient should be on a diet consisting of 50-150 g of fat per day for 3 days prior to the study. Non-absorbable fat substitutes such as olestra should be avoided prior to collection. When multiple tests are ordered, submit separate sample for this test.Specimens not submitted in pre-weighed container, samples >500 mL or >500 g, paint cans, random collections, samples containing barium or charcoal, samples submitted in media or preservatives.

 

O&P with formalin for collection of stool
Test NameTransport TempSpecimen RequirementsRejection Criteria
Ova and Parasites with Trichrome StainRoom Temperature (15-25 °C)Stool submitted in Formalin and PVA preservative. Code appropriate for 1-3 sets. For urine specimens, please order CPL code 7004 - parasite exam, urine.Frozen stool, leaking containers, contaminated specimens, specimens with quantity not sufficient for testing, swabs or unpreserved stool, diapers or tissue paper.
Parasite StainRoom Temperature (15-25 °C)1. Stool sample should be collected in a clean, wide-mouth container, bedpan or on a clean newspaper or plastic bag placed over the toilet seat opening. Do not pass the specimen directly into the toilet or directly into the collection vial. Do not mix urine or water with the specimen. 2. Open the vial carefully. 3. Using the collection spoon attached to the cap, add enough specimen until the liquid reaches the arrow on the label. 4. Sample areas of the specimen which appear bloody, slimy or watery. If stool is hard, sample from each end and the middle of the specimen. No more than three (3) parasitology specimens are recommended over 7-10 days because shedding may be intermittent. Three (3) specimens will pick up 99% of parasitic pathogens. Do not use this test for microspora stain (order CPL code 7006).Frozen stool, leaking containers, contaminated specimens, specimens with quantity not sufficient for testing, swabs or unpreserved stool, diapers or tissue paper.
A test of fecal occult blood includes three stir sticks and three fecal occult labels as wll as a sell addressed return envelope
Test NameTransport TempSpecimen RequirementsRejection Criteria
Occult Blood, Screen (1-3)

Ambient (15-25 °C)

At least one marble-sized portion of fresh stool or inoculated hemoccult slide. Diet recommended: avoid rare meats, broccoli, horseradish, cauliflower, cantaloupe, parsnip, red radishes, rectal mediations, and supplements containing >250 mg Vitamin C.Samples submitted in preservative, samples other than stool or colon washings over-inoculated cards or those inoculated on the wrong side.

Occult Blood, Diagnostic (1-3)

Ambient (15-25 °C)

At least one marble-sized portion of fresh stool or inoculated hemoccult slide. Diet recommended: avoid rare meats, broccoli, horseradish, cauliflower, cantaloupe, parsnip, red radishes, rectal medications, and supplements containing >250 mg Vitamin C.Samples submitted in preservative, samples other than stool or colon washings over-inoculated cards or those inoculated on the wrong side.
Polymedco OC Auto
Test NameTransport TempSpecimen RequirementsRejection Criteria
Occult Blood, Immunoassay Diagnostic

Room Temperature (15-25 °C)

Fresh fecal sample in Polymedco green cap collection paddle. Only 1 sample is required. There are no dietary restrictions.Frozen samples, samples other than stool, samples not received in green Polymedco sampling device, samples received in broken containers or grossly contaminated samples.

 Occult Blood, Immunoassay MC Screen

Room Temperature (15-25 °C)

Fresh fecal sample in Polymedco green cap collection paddle. Only 1 sample is required. There are no dietary restrictions.Frozen samples, samples other than stool, samples not received in green Polymedco sampling device, samples received in broken containers or grossly contaminated samples.